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Having said that, the extent of hydrolysis in the freeze-dried fish roe homogenates via the 3 enzymes was lessen than that accomplished to the roe treatments as mentioned above, which can be related to adjustments from the protein composition brought on by the freeze drying, which resulted in very low enzyme-substrate contact performance [45]. Dehydration by heating or lyophilization can lead to protein denaturation. These kinds of alterations could modify the secondary, tertiary, or quaternary buildings of your protein molecules [forty six]. Indications of protein denaturation are typically observed as modification of their solubility.
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